𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites

✍ Scribed by Korenori Ogata; Satoshi Imazato; Atsushi Ehara; Shigeyuki Ebisu; Yoshifumi Kinomoto; Takayoshi Nakano; Yukichi Umakoshi

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
999 KB
Volume
72A
Category
Article
ISSN
1549-3296

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Hydroxyapatite/soluble calcium phosphate composites (HAp/SCaP) are novel HAp‐based materials with enhanced solubility that have been developed by annealing HAp in a vacuum. This study compared the effects of HAp and HAp/SCaP on osteoblast proliferation, differentiation, and mineralization using an MC3T3‐E1 cell culture system. MC3T3‐E1 cells were cultured on HAp or HAp/SCaP, and the number of attached cells and their morphology were examined. The influence of the extract from HAp/SCaP on osteoblast differentiation was determined by the measurement of alkaline phosphatase activity and reverse transcriptase‐polymerase chain reaction analysis of the expression of osteoblastic markers. In addition, mineralization was evaluated by the staining of calcium deposits with Alizarin red. Attachment of a greater number of cells exhibiting no degeneration in their morphology was observed on HAp/SCaP compared with HAp after incubation for 7 days or more. Culturing cells with the extract from HAp/SCaP resulted in promotion of alkaline phosphatase activity, the expression of type I collagen, and bone‐like tissue formation. The results of the present study indicate that HAp/SCaP shows greater ability in osteogenesis than HAp by increasing collagen synthesis and calcification of the extracellular matrix. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 72A: 127–135, 2005

📜 SIMILAR VOLUMES

Comparison of osteoblast-like cell respo
Comparison of osteoblast-like cell responses to calcium silicate and tricalcium phosphate ceramics in vitro
✍ Siyu Ni; Jiang Chang; Lee Chou; Wanyin Zhai 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 778 KB

## Abstract Calcium silicate ceramics have been proposed as new bone repair biomaterials, since they have proved to be bioactive, degradable, and biocompatible. β‐tricalcium phosphate ceramic is a well‐known degradable material for bone repair. This study compared the effects of CaSiO~3~ (α‐, and β

Mechanical performance and osteoblast-li
Mechanical performance and osteoblast-like cell responses of fluorine-substituted hydroxyapatite and zirconia dense composite
✍ Hae-Won Kim; Jonathan C. Knowles; Long-Hao Li; Hyoun-Ee Kim 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 649 KB

## Abstract A fluorine‐substituted hydroxyapatite (FHA) and zirconia (ZrO~2~) dense composite (50:50 by volume) was fabricated, and its feasibility for hard tissue applications was investigated in terms of its mechanical properties and osteoblast‐like cell (MG63) responses __in vitro__. The incorpo

ChemInform Abstract: Synthesis of Calciu
ChemInform Abstract: Synthesis of Calcium Hydroxyapatite—Tricalcium Phosphate (HA—TCP) Composite Bioceramic Powders and Their Sintering Behavior.
✍ N. KIVRAK; A. C. TAS 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 34 KB 👁 1 views

Synthesis of Calcium Hydroxyapatite-Tricalcium Phosphate (HA-TCP) Composite Bioceramic Powders and Their Sintering Behavior. -The HA-TCP composite powders are synthesized, for the first time, from aqueous solutions by a novel, one-step chemical precipitation technique. The starting materials consis

Biomineralization of calcium phosphate o
Biomineralization of calcium phosphate on human hair protein film and formation of a novel hydroxyapatite–protein composite material
✍ Toshihiro Fujii; Teppei Tanaka; Kousaku Ohkawa 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 344 KB

## Abstract Human hair protein can be used not only as a totally biodegradable material but also as a “self‐originated” material, which may avoid an undesirable immune reaction, if it has been prepared from a certain individual and implanted into the same person. In this study, a novel organic–inor

X-ray determination of crystalline hydro
X-ray determination of crystalline hydroxyapatite to amorphous calcium-phosphate ratio in plasma sprayed coatings
✍ Keller, Ludwig ;Dollase, W. A. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 188 KB 👁 2 views

A method to measure the amorphous calcium phosphate (ACP) content of plasma sprayed hydroxyapatite (HA) coatings is presented. The areas of the crystalline peaks and the broad amorphous "hump" in an X-ray diffraction scan of a mixture of crystalline and amorphous HA can be fitted using conventional

In vivo response of porous hydroxyapatit
In vivo response of porous hydroxyapatite and β-tricalcium phosphate prepared by aqueous solution combustion method and comparison with bioglass scaffolds
✍ Samir K. Ghosh; Samit K. Nandi; Biswanath Kundu; Someswar Datta; Dipak K. De; Su 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 555 KB

## Abstract Pure hydroxyapatite (HAp) and a biphasic calcium phosphate [containing 90% of β‐tri‐calcium phosphate (β‐TCP) and 10% HAp] were tailored through an aqueous solution combustion synthesis. Porous struts were prepared using all the powders along with bioglass, a known bioactive material, a