Fetal erythroblasts circulating in maternal blood are important candidate cells for non-invasive prenatal diagnosis. We have cultured erythroblasts from 16 maternal blood samples, both with and without prior enrichment by magnetic activated cell sorting (MACS), in a semi-solid medium containing grow
โฆ LIBER โฆ
Comparison of methods for erythroblast selection: Application to selecting fetal erythroblasts from maternal blood
โ Scribed by Ellen J. Collarini; Carol A. Cain; Dawn Gammon; Bill Harriman; Kerry Magee; Gehua Du; Paula A. Schueler; Walt C. Mahoney
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 310 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0196-4763
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In order to detect fetal nucleated red blood cells (NRBCs) in maternal blood, a protocol was developed which aimed at producing a reliable staining method for combined immunocytochemical and FISH analysis. The technique had to be suitable for eventual automated screening of slides. Chorionic villi w