Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme-Linked Immunosorbent Assay Employing Chicken Yolk Antibodies

✍ Scribed by Sona Krizkova; Pavlina Blahova; Johana Nakielna; Ivo Fabrik; Vojtech Adam; Tomas Eckschlager; Miroslava Beklova; Zdenka Svobodova; Vratislav Horak; Rene Kizek

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 452 KB
• Volume: 21
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200900243

✦ Synopsis

Abstract

In this study, two methods for metallothioneins (MT) determination in a biological sample were compared. Particularly, twenty five human and nine pig blood serum samples and liver and kidney samples from thirty five carps (Cyprinus carpio) were analyzed by enzyme‐linked immunosorbent assay and differential pulse voltammetry Brdicka's reaction. The results obtained by these two methods were in good agreement. For commercially available MT standard the correlation coefficient between the single concentrations signal height was 0.99. In biological samples the correlation coefficients were 0.90 for fish liver and kidney samples, 0.91 for pig blood serum and 0.93 for human blood serum.