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Comparison of Matrigel™ and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing

✍ Scribed by A.R. Greenlee; T.A. Kronenwetter-Koepel; S.J. Kaiser; K. Liu


Book ID
116919244
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
274 KB
Volume
19
Category
Article
ISSN
0887-2333

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## Abstract The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA