Comparison of implantation and cytotoxicity testing for initially toxic biomaterials

✍ Scribed by Agneta Rosengren; Linda Faxius; Noriho Tanaka; Mika Watanabe; Lars M. Bjursten

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 355 KB
• Volume: 75A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.30431

✦ Synopsis

Abstract

To evaluate the predictive value of cytotoxicity testing, the present study compares the in vivo tissue responses to in vitro cytotoxicity before and after implantation. Material toxicity was caused by addition of the toxic substance Zincdiethyldithiocarbamate (ZDEC) that is used as a standard for in vitro cytotoxicity testing. Polyurethane discs with the addition of 0.5% or 1% ZDEC as well as nontoxic discs were inserted in the abdominal wall of rats for 1 day up to 6 weeks. After explantation the foreign body response was analyzed immunohistochemically. An in vitro reanalysis of the explanted reference materials (RMs) revealed remaining high concentrations of toxic compounds after 1‐week implantation, whereas no toxicity was seen after 6 weeks implantation. This was reflected in the foreign body response where a significantly thicker capsule and more inflammatory cells were seen at 1 week for the toxic implants. Over time, with decreasing toxicity, these differences disappeared. Test samples that only were subjected to in vitro extraction with water did not elute toxic compounds to the same extent as the in vivo conditions. It is concluded that many clinically useful implant materials may be unnecessarily rejected due to the results of in vitro tests. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005