Comparison of Immunoassays for Reproduction- and Thyroid-Hormones Performed on Five Automated Analysers: ARCHITECT, AxSYM, Centaur, Elecsys 2010 and IMMULITE 2000 : Methodenvergleich von Immunoassays für Sexual- und Schilddrüsenhormone von fünf Routineanalysern: ARCHITECT, AxSYM, Centaur, Elecsys 2010 und IMMULITE 2000
✍ Scribed by M. Veitl; A. Hamwi; Andrea Huber; J. Flores; R. Dudczak; C. Bieglmayer
- Book ID
- 114430465
- Publisher
- Walter de Gruyter GmbH & Co. KG
- Year
- 2002
- Tongue
- German
- Weight
- 391 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0342-3026
No coin nor oath required. For personal study only.
✦ Synopsis
Efficacy data as well as correlations of test results measured by different automated immunoassay analysers are obligatory for organisational and medical decisions on a rational scientific basis. Thus, we compared the serum concentrations of reproductive hormones (lutropin, follitropin, estradiol, progesterone, testosterone and prolactin) measured by automated immunoassay-procedures performed on ARCHITECT (Abbot-Laboratories, USA), Elecsys 2010 (Roche-Diagnostics, Switzerland) and IMMULITE 2000 (Diagnostic Products Corporation, USA). Similarly, concentrations of hormones for the estimation of thyroid function (thyreotropin, thyroxin, free thyroxin, triiodothyronine and free triiodothyronine) were measured by these analysers and in addition by AxSYM (Abbot-Laboratories, USA) and ACS:Centaur (Bayer, Germany). At low hormone concentrations we observed for all investigated immunoassays a low day-to-day precision, which might be clinically relevant especially for testosterone and estradiol measurements in women and children. The different assays for reproduction-hormones generally showed a high correlation, whereby a higher scattering of data around the regression lines was observed in the lower measuring range. However, IMMU-LITE 2000 measured higher testosterone concentrations than the other methods. Differences between results obtained by diverse lutropin and prolactin assays were apparently due to different test calibrations. Most automated thyroid-hormone assays correlated quite well. Higher levels for free triiodothyronine were, however, measured by Elecsys 2010.
Although results from the new generation of automated immunoassay-analysers showed some improvements regarding intermethod comparability, it seems still partly unavoidable to establish method specific reference ranges.