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Comparison of IMAC and MOAC for phosphopeptide enrichment by column chromatography

✍ Scribed by Luc Negroni; Stephane Claverol; Jean Rosenbaum; Eric Chevet; Marc Bonneu; Jean-Marie Schmitter


Book ID
116620751
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
397 KB
Volume
891-892
Category
Article
ISSN
1873-376X

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## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the in‐depth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig