Comparison of gibberellin-like substances from cotyledons and phloem exudate collected from cotyledons ofPharbitis nilin relation to photoperiodic flowering
✍ Scribed by Kiyotoshi Takeno; Charles F. Cleland
- Book ID
- 104621503
- Publisher
- Springer Netherlands
- Year
- 1990
- Tongue
- English
- Weight
- 391 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0167-6903
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✦ Synopsis
Gibberellin (GA)-like substances were analyzed in extracts from cotyledons and phloem exudate collected from cotyledons in photoinduced and vegetative seedlings of the short-day plant Pharbitis nil Chois . var . Violet, using high performance liquid chromatography (HPLC) and the dwarf rice bioassay, to see whether any specific GA-like substances were transported from the photoinduced cotyledons via phloem . Cotyledon extracts exhibited five peaks of free GA-like activity in HPLC, whereas only one or two active peaks were detected in phloem exudate extracts . The level of free GA-like activity was considerably lower in phloem exudate than in the cotyledons . In five out of six analyses of cotyledons and phloem exudate, there were substantially higher levels of free GA-like substances in photoinduced plants . Conjugated GA-like substances were present in much higher levels than free GA-like substances in the cotyledon extracts but the levels were not influenced by daylength . In phloem exudate extracts there was no conjugated GA-like substances . The free GA-like substances that are transported via phloem cochromatographed with GA, / , and GA 19 on HPLC . These were significantly higher in photoinduced plants and thus could have some influence on the photoperiodically-induced flowering in P. nil .