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Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells

โœ Scribed by Tomoyuki Yamaguchi; Azusa Morikawa; Hiroyuki Miyoshi


Book ID
116303778
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
753 KB
Volume
425
Category
Article
ISSN
0006-291X

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Special vectors have been constructed that allow the trapping of genes in mouse embryonic stem (ES) cells. These vectors generally contain the neomycin phosphotransferase (neo) gene for selection and the โค -galactosidase (โค-gal) gene as a marker. Promoterless vectors can be used to identify genes th