Comparison of Fluorescence, 31P NMR, and 7Li NMR Spectroscopic Methods for Investigating Li+/Mg2+ Competition for Biomolecules

✍ Scribed by Louis Amari; Brian Layden; Qinfen Rong; Carlos F.G.C. Geraldes; Duarte Mota de Freitas

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 155 KB
• Volume: 272
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1999.4169

✦ Synopsis

2 Abbreviations used: G-proteins, guanine nucleotide-binding proteins; K d , dissociation constant; RBC, red blood cells; R max , fluorescence intensity ratio at 335 and 370 nm in presence of saturating amounts of Mg 2ϩ ; RЈ max , intensity ratio in the presence of saturating amounts of Li ϩ ; R min , intensity ratio in the absence of metal ions; S max , fluorescence intensity at 370 nm in the presence of saturating amounts of Mg 2ϩ ; SЈ max , fluorescence intensity at 370 nm in the presence of saturating amounts of Li ϩ ; S min , fluorescence intensity at 370 nm in the absence of metal ions; T 1 , spin-lattice relaxation measurement.