Comparison of dynamically coated SE-54, SP-2250 and carbowax 20M wall-coated open tubular (WCOT) glass capillary columns, prepared after surface pretreatment with Superox™-4 or with BaCO3
✍ Scribed by Arrendale, R. F. ;Smith, L. B. ;Rogers, L. B.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 619 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
A new surface pretreatment for the preparation of wall‐coated open tubular (WCOT) glass capillary columns has been evaluated. This technique involves the application of a non‐extractable layer of Superox™‐4, a 4,000,000 MW polyethylene glycol, to the glass surface as a pretreatment and deactivation agent. Unlike other polyethylene glycols, Superox‐4 is stable at high temperatures (> 300°C) in the absence of oxygen, coats smoothly onto a bare glass surface and resists droplet formation. WCOT columns (SE‐54, Carbowax 20M, and SP‐2250) prepared using this technique are compared to columns prepared using a modified Grob [2,3] BaCO~3~ procedure. The Superox‐4 pretreated columns were equal or superior in quality to the BaCO~3~ pretreated columns, based on the appearance of an activity standard and the effective theoretical plates (N~eff~) per meter. Chromatograms showing practical application of the WCOT columns prepared using both methods is given.