Comparison of desmoglein ELISA and indirect immunofluorescence using two substrates (monkey oesophagus and normal human skin) in the diagnosis of pemphigus

The use of two substrates to improve the

The use of two substrates to improve the sensitivity of indirect immunofluorescence in the diagnosis of pemphigus

✍ K.E. Harman; M.J. Gratian; B.S. Bhogal; S.J. Challacombe; M.M. Black 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 314 KB

## Abstract The aim of this study was to re-evaluate indirect immunofluorescence (IIF) comparing two substrates, normal human skin (HS) and monkey oesophagus (MO) using serum from 29 pemphigus patients classified according to the presence of serum autoantibodies to either desmoglein (Dsg) 1 or Dsg3