✦ LIBER ✦

Comparison of cathepsin d determinations in human carcinomas by enzyme immunoassay and immunoradiometric assay

✍ Scribed by Raymond M. Shaheen; Slobodan Miseljic; David L. Doering; Dr. James L. Wittliff

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 566 KB
Volume: 9
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860090604

No coin nor oath required. For personal study only.

✦ Synopsis

Overexpression of cathepsin 0 in several types of carcinoma in women appears to be associated with a poor clinical course. In this prospective investigation, cathepsin D levels in 170 specimens of normal and neoplastic human tissues were determined simultaneously by enzyme immunoassay (EIA) and immunoradiometric assay (IRMA) to allow comparisons in multicentric studies, such as cooperative clinical trials. Nonmalignant uteri and specially prepared reference powders were also evaluated. Linear regression analysis between the two assays for all specimens [EIA = 0.87(IRMA) -3.181 demonstrated a correlation coefficient (r) of 0.99 ( P < 0.001). When malignancies were categorized by the tissue origin (i.e., breast, uterus, ovary, lymph node, and colon), highly significant correlations were also observed (regressions slopes ranged from 0.58 to 1.02). Intra-and interassay controls conducted for the new EIA procedure gave CV% ranging from 4.4 to 10.2, which was similar to the IRMA test for cathepsin D. The results of both assays correlated well and were highly reproducible. Either assay may be used with confidence that comparable cathepsin D values will be obtained in a wide range of tissue biopsies. o 1995 Wiley-Liss, Inc.

📜 SIMILAR VOLUMES

Comparison of sex steroid receptor deter

Comparison of sex steroid receptor determinations in human breast cancer by enzyme immunoassay and radioligand binding

✍ Resad Pasic; Benjamin Djulbegovic; James L. Wittliff 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 630 KB

Most investigators comparing ligand-binding procedures for quantifying estrogen and progestin receptors in human breast cancer with procedures employing monoclonal antibodybased methods have utilized an inappropriate variety of reaction conditions, including the elimination of sodium molybdate in th

Comparison of an inhalant allergy immuno

Comparison of an inhalant allergy immunoassay with allergen specific IgE determinations in human serum

✍ Thomas B. Martins; Christine M. Litwin; Troy D. Jaskowski; Harry R. Hill 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 33 KB

The diagnosis of inhalant allergy can be elusive, with symptoms resembling viral or bacterial infection, as well as immunologic deficiency. In this study an inhalant allergy immunoassay was investigated as a possible screen to rule in or out respiratory inhalant allergy in patients with allergictype

Reappraisal of p53 mutations in human ma

Reappraisal of p53 mutations in human malignant astrocytic neoplasms by p53 functional assay: Comparison with conventional structural analyses

✍ Mitsuhiro Tada; Richard D. Iggo; Francois Waridel; Michimasa Nozaki; Ryoji Matsu 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 282 KB 👁 1 views

We previously reported clonal expansion of p53 mutations in malignant astrocytic tumors detected with a yeast p53 functional assay that measures mutant p53 alleles quantitatively and loss of p53 transcriptional competence qualitatively (Tada et al., Int J Cancer 67:447-450, 1996). This method select

Comparison of estrogen receptor determin

Comparison of estrogen receptor determinations by a biochemical ligand-binding assay and immunohistochemical staining with monoclonal antibody ER1D5 in females with lymph node positive breast carcinoma entered on two prospective clinical trials

✍ Steven R. Alberts; James N. Ingle; Patrick R. Roche; Stephen S. Cha; Lester E. W 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 830 KB

## BACKGROUND. The measurement of estrogen receptors (ER) in breast cancer specimens has traditionally been assessed with a tlextran-coated charcoal assay (DCCA). More recently the immunohistochemical staining (IHC) method has gained increasing popularity because of its ability to use fixed tissue