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Comparison of cAMP-dependent protein kinase substrate specificity in reaction with proteins and synthetic peptides

✍ Scribed by Mart Loog; Nikita Oskolkov; Fergal O'Farrell; Pia Ek; Jaak Järv

Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
148 KB
Volume
1747
Category
Article
ISSN
1570-9639

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✦ Synopsis

Mutants of L-type pyruvate kinase with modified peptide sequence around the Ser-12 phosphorylation site were prepared and kinetics of their phosphorylation by protein kinase A was studied. The profile of substrate specificity obtained for these proteins was compared with the kinetic data of phosphorylation of short peptide substrates. Alterations made in protein structure caused weaker effects than the corresponding alterations made in peptides, while the amino acid preferences and the overall specificity pattern remained similar in the both cases. Thus, similar consensus motif holds for both protein and peptide substrates, but is less critical for recognition of proteins if compared with short peptides.

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