Comparison of antibody responses to different forms of HIV-1 core antigens by epitope mapping
✍ Scribed by Truong, Catherine; Brand, Denys; Mallet, François; Roingeard, Philippe; Barin, Francis
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 260 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
The specificity of antibodies to HIV-1 capsid (p24CA) and matrix (p17MA) proteins, produced in mice against unprocessed immature assembled polyprotein (wild-type p55 virus-like particles or chimeric p55 virus-like particles) or against the monomeric mature form (rp24CA/rp17MA), was analyzed by a microplate epitope mapping assay using a panel of synthetic peptides covering the entire p24CA plus p17MA sequences of HIV-1LAI. All immunized mice developed anti-p24CA and anti-p17MA antibodies, although the spectrum of specificity of these antibodies was different. Four p24 CA epitopes (residues 176-192, 201-218, 233-253, 285-304) were recognized by anti-rp24CA/rp17MA antibodies, whereas one p17MA epitope (residues 11-25) and one p24CA epitope (residues 176-192) were constantly recognized by anti-p55 virus-like particle antibodies. These results suggest a different specificity pattern of anti-p24CA and anti-p17MA antibodies depending on whether they are produced against the soluble mature form or the immature assembled form of the gag proteins.