Comparison of analytically and numerically derived hydrogen exchange-rate distribution functions

✍ Scribed by Roger B. Gregory

• Book ID: 102762499
• Publisher: Wiley (John Wiley & Sons)
• Year: 1983
• Tongue: English
• Weight: 746 KB
• Volume: 22
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360220311

✦ Synopsis

Hydrogen exchange-rate probability density functions for lysozyme have been derived by numerical Laplace inversion with the computer program CONTIN. The resulting solution set includes a smooth bimodal solution in agreement with previous analytical results together with a smooth three-peak solution. Numerical analysis of lysozyme hydrogen-exchange data in glycerol/water cosolvent mixtures confirms the previous assignment of the slow-exchange peak to an exchange mechanism involving reversible unfolding. Physicochemical constraints that can reduce the size of the solution set are described. The results are compared with those obtained from previous analytical methods and the limitations of the discrete class and analytical approaches are discussed.