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Comparison of analytical methods for extraction of chloride from plant tissue using36Cl as tracer

✍ Scribed by G. Ghosh; M. C. Drew


Book ID
105478281
Publisher
Springer
Year
1991
Tongue
English
Weight
297 KB
Volume
136
Category
Article
ISSN
0032-079X

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✦ Synopsis


Eleven published and unpublished methods for extraction of chloride from plant tissue were compared, using a homogeneous sample of dried, ground maize leaves previously labelled with 36C1. The most effective method of extraction of CI-, estimated by liquid scintillation counting of 36C1, was with hot water. However, six other methods, including either cold water extraction, or dry-ashing at 500~ of plant material previously treated to give an alkaline pH, gave 36C1 values that were not statistically different from that obtained with hot water extraction. The lowest estimates of 36C1 content were obtained either by wet digestion in nitric/acetic acid or by dry-ashing following Mg(NO3) 2 treatment of the plant material.


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