Comparison of a novel ColiPlateTM kit and the standard membrane filter technique for enumerating total coliforms andEscherichia coli bacteria in water
✍ Scribed by Lifshitz, Ran ;Joshi, Renu
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 159 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1053-4725
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✦ Synopsis
The new ColiPlate CP kit was evaluated comparatively with the standard membrane filter ( ) MF technique for enumerating total coliforms and Escherichia coli in water. In testing natural water
( 2 ) samples, good correlations were observed for enumerating total coliforms R = 0.84 and E. coli ( 2 ) R = 0.95 . However, counts of E. coli population density estimated by CP were 47% higher than counts estimated by MF. With the water samples spiked with culture-grown E. coli cells, the correlation
( 2 ) ( 2 ) between the methods was strong for both total coliforms R = 0.95 and for E. coli R = 0.94 . E. coli densities were estimated to be 20% higher using CP compared with MF. Samples spiked with ( ) rehydrated freeze-dried E. coli cells with high portion of injured or weakened cells showed a strong
( 2 ) correlation between the two methods R = 0.93, for either total coliforms or E. coli . However, estimated ( ) total coliforms and E. coli densities were higher by CP than MF counts 38 and 168%, respectively . The CP test is therefore considered a more reliable method than the traditional MF for enumerating E. coli in samples with high levels of injured or weakened cells.