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Comparison of a cytosol radioreceptor assay with a radioimmunoassay for 1,25-dihydroxyvitamin D in serum or plasma

✍ Scribed by AndréP. De Leenheer; Rita M. Bauwens


Book ID
115825071
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
832 KB
Volume
152
Category
Article
ISSN
0009-8981

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Cytosol prepared from small intestine of vitamin D-sufficient rabbits contains a specific highaffinity binding protein for 1,25dihydroxyvitamin D, (1,2S(OH),Ds). This binding protein sediments at 3.0-3.5 S in sucrose. density gradients containing 0.3 M KCI. Scatchard analysis using intestinal cytoso