Comparison between the polymerase chain reaction and slot blot hybridization for the detection of HPV sequences in cervical scrapes
β Scribed by P. WARD; G. N. PARRY; R. YULE; D. V. COLEMAN; A. D. B. MALCOLM
- Book ID
- 108705596
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 256 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0956-5507
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Cervical scrapes from 80 women with a positive cytology result were tested for the presence of human papillomavirus (HPV) using the polymerase chain reaction (PCR) and compared to the results obtained with the modified filter in situ hybridisation (FISH) and the Southern-blot techniques. The sensit
## Abstract A newly developed general primerβmediated polymerase chain reaction (GPβPCR) was used for the detection of a broad spectrum of Human Papillomaβvirus (HPV) genotypes, including unsequenced types, in cytologically normal and abnormal cervical smears and in biopsies of cervical carcinomas.