Comparison between collision induced dissociation of electrosprayed protonated peptides in the up-front source region and in a low-energy collision cell
✍ Scribed by W. D. van Dongen; J. I. T. van Wijk; B. N. Green; W. Heerma; J. Haverkamp
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 66 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0951-4198
No coin nor oath required. For personal study only.
✦ Synopsis
A systematic comparison between the up-front Collision induced dissociation (CID) mass spectra and low-energy CID tandem mass spectra from twenty-one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up-front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up-front CID and low-energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated.