✦ LIBER ✦

Comparison between a LAPS and an FET-based sensor for cell-metabolism detection

✍ Scribed by A Fanigliulo; P Accossato; M Adami; M Lanzi; S Martinoia; S Paddeu; M.T Parodi; A Rossi; M Sartore; M Grattarola; C Nicolini

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 547 KB
Volume: 32
Category: Article
ISSN: 0925-4005
DOI: 10.1016/0925-4005(96)80107-9

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✦ Synopsis

Several silicon-based biosensors have been developed for various applications, such as enzymatic and immunological activity determination and cell-metabolism detection. This work describes an electrochemical characterization of ion-sensitive field-effect transistor (1SFET) and light-addressabie potentiometri¢ sensor (LAPS) transducers and a test of such devices as detectors of 3T6 (Swiss albino mouse embryo, fibroblasts) metabolism. In particular, our investigation is devoted to some fundamental parameters of these transducers (e.g., pH sensitivity, drift, temperature dependence of the output signal, speed of response) for the purpose of comparing their performance related to cellmetabolism evaluation. The final goal is therefore to analyze the capabilities of these silicon-based transducers for use in a biosensing system for cell-metabolism detection.

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