Comparing qualitative and quantitative spectroscopic techniques for the detection of the effect of direct iron loading of mammalian cell cultures

A Spectrophotometric Method for the Dire

A Spectrophotometric Method for the Direct Detection and Quantitation of Nitric Oxide, Nitrite, and Nitrate in Cell Culture Media

✍ Lisa A. Ridnour; Julia E. Sim; Michael A. Hayward; David A. Wink; Sean M. Martin 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 117 KB

A method for the spectrophotometric determination of nitric oxide, nitrite, and nitrate in tissue culture media is presented. The method is based on the nitric oxide-mediated nitrosative modification of sulfanilic acid that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride forming an orange-