Comparative ultrasound evaluation of human trabecular bone graft properties after treatment with different sterilization procedures
✍ Scribed by L. Vastel; C. Masse; P. Mesnil; E. Crozier; F. Padilla; P. Laugier; D. Mitton; J. P. Courpied
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 122 KB
- Volume
- 90B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
New sterilization methods for human bone are likely to affect the mechanical properties of human cancellous grafts. These mechanical properties dictate the short‐ and mid‐term results of the orthopedic procedure. The aim of this study was to compare the effects on bone mechanical properties, as assessed by ultrasound velocity, of different sterilization methods used under similar conditions: bleach and sublimation, humid heat, successive baths of physiological saline with osmotic detersion, and CO~2~ in the supercritical phase. Alterations in mechanical properties were small with CO~2~ (velocity change: −2%) and humid heat (−2.5%). Osmotic detersion had a significant but moderate effect (−4.7%). The −9% change with the protocol involving bleach suggested a greater than 30% decrease in load to failure, based on earlier studies. Gamma irradiation of defatted trabecular allografts, in a dose of 10 or 25 KGy, produced no significant changes in ultrasound velocity. Powerful protein denaturants used in sterilization protocols substantially alter the mechanical resistance of the grafts, which may jeopardize the orthopedic procedure. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009