Comparative targeting of human colon-carcinoma multicell spheroids using one- and two-step (bispecific antibody) techniques
✍ Scribed by Anne Devys; Philippe Thedrez; Emmanuel Gautherot; Alain Faivre-Chauvet; Catherine Saï-Maurel; Eric Rouvier; Jean-Louis Auget; Jacques Barbet; Jean-François Chatal
- Book ID
- 102653667
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 781 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
In the perspective of radioimmunotherapy (RIT) of micrometastases, we compared, in multicell spheroids (MS), the uptake and retention kinetics of '2sl-F(ab)~ F6 anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb), and the affinity enhancement system (AES) using an anti-CEA/anti-DTPAindium bispecific antibody (BsMAb) and a 12SI-labeled di-DTPA-
In-tyrosine-lysine bivalent hapten. We used MS of colorectaltumor cell lines expressing CEA strongly (LS 174T). weakly (HT-29) or not at all HRT-18). Uptake and retention kinetics of
The highest uptake values, obtained with LS 174T MS, were slightly lower with AES than with I2%F(ab); F6. However, effective retention half-lives were longer for AES than for 1251-F(ab); F6 or for I "In-labeled monovalent hapten after pre-incubation of spheroids with BsMAb. Autoradiography showed the same slow and heterogeneous distribution of 1251-F(ab); F6 and '2sl-BsMAb. These results indicate that the 2-step technique is more favorable for R I T uptake values were approximately the same but uptake kinetics were more rapid, and retention half-life was longer than with the one-step technique.