Comparative study on the inclusion behaviour of cyclodextrin derivatives with venoruton and rutin by thin layer chromatography
✍ Scribed by Xiliang Guo; Shaomin Shuang; Xiaoping Wang; Chuan Dong; Jinghao Pan; Hassan Y. Aboul-Enein
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 216 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0269-3879
- DOI
- 10.1002/bmc.356
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✦ Synopsis
The interaction of rutin and venoruton (troxerutin), with α-, β -and γ-cyclodextrin (CD), hydroxypropyl-βcyclodextrin (HP-β -CD) and methyl-β -cyclodextrin (M-β -CD) was investigated by reversed-phase thin layer chromatography on polyamide plates. A mobile phase consisted of NH 4 OH; NH 4 Cl buffer solution containing various CD concentrations (pH = 9.7, 20°C) was used as mobile phase. The equilibrium constants (K f ) and the retention factor (R f ) were determined and used to study the inclusion process. The influence of CDs on the solubility of rutin and venoruton was characterized by R M values and the increasing hydrophilicity of drugs. The results show that the inclusion capacity of cyclodextrins follows the order HP-β-CD > M-β -CD > β -CD > γ -CD, and rutin is more easily included by the studied cyclodextrins than venoruton. In addition, the thermodynamic parameters (∆H, ∆S) for the formation of complexes were obtained from the van't Hoff equation, displaying the enthalpyentropy compensation effect.