Comparative study on the ALA photodynamic effects of human glioma and meningioma cells

✍ Scribed by Tsai, Jui-Chang; Hsiao, Yi-Yun; Teng, Lee-Jene; Chen, Chin-Tin; Kao, Ming-Chien

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 221 KB
• Volume: 24
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/(sici)1096-9101(1999)24:4<296::aid-lsm7>3.0.co;2-f

✦ Synopsis

Background and Objective:

The purpose of this study was to compare the differential susceptibility to photodynamic therapy (PDT) mediated damage in human U-105MG glioma cells and CH-157MN meningioma cells in vitro using 5-aminolevulinic acid (ALA) as photosensitizer, and to determine if growth factors would enhance PDT-mediated damage of these cells. Study Design/Materials and Methods: U-105MG or CH-157MN cells were irradiated with polychromatic light in the presence of ALA. A Xenon lamp (150 W) was used as the light source. For the study on the effect of growth factor on ALA-PDT, cells were cultured in serum free medium for 24 hours. Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), or platelet derived growth factor BB (PDGF-BB) was added to achieve a final concentration of 50 ng/ml. 30 minutes later, cells were incubated with ALA (100 g/ml) for 24 hours, washed, and irradiated with light (11 J/cm 2 ). MTT tetrazolium assays were performed 24 hours after light irradiation. Results: The inhibition of metabolic cellular function in U-105MG cells by ALA depended on both light energy density and ALA concentration. The susceptibility to ALA-PDT was profoundly lower for CH-157MN meningioma cells than U-105MG glioma cells. When incubated with ALA (100 g/ml), U-105MG cells exhibited an LD 50 around 8 J/cm 2 of light irradiation, whereas that of CH-157MN cells was more than 25 J/cm 2 . EGF, bFGF, or PDGF-BB did not have any effects on the susceptibility of these two cell lines to ALA-PDT. Conclusion: ALA-PDT was more effective in killing U-105MG glioma cells than CH-157MN meningioma cells. The differential susceptibility was likely due to differential accumulation of PpIX in these cells. EGF, bFGF, or PDGF-BB did not have stimulatory or inhibitory effect on the efficiency of ALA-PDT. Lasers