Comparative study on Lewis lung tumor lines with ‘low’ and ‘high’ metastatic capacity. II. Cytochemical and biochemical evidence for differences in glycosaminoglycans

✍ Scribed by J. Timárf; E. Móczar; O. Timár; K. Pál; L. Kópper; K. Lapis; A. Jeney

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 569 KB
• Volume: 5
• Category: Article
• ISSN: 0262-0898
• DOI: 10.1007/bf00116628

✦ Synopsis

The enhanced metastatic capacity of an in vivo selected Lewis lung tumor line (LLT-HH) was correlated with changes in cell-associated glycosaminoglycans (GAG) using ultrastructural cytochemistry, flow cytometry and biochemistry. The increase in highly sulphated GAG content on the cell membrane of LLT-HH cells compared to the parent LLT cells was demonstrated cytochemically. Using in vitro [3H]glucosamine labelling of GAG components it was shown that the 1.I.T-HH cells were characterized by a high production of heparan sulphate while the parent LLT line had a high hyaluronic acid~:hondroitin sulphate production. The high metastatic phenotype is accompanied by an altered production of cell-associated GAGs.