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Comparative study of the antimutagenic properties of vitamins C and E against mutation induced by norfloxacin

✍ Scribed by Myriam Arriaga Alba; Roberto Rivera Sánchez; Nancy Jannet Ruíz Pérez; Jaime Sánchez Navarrete; Rocío Flores Paz; Araceli Montoya-Estrada; Juan José Hicks Gómez

Publisher
BioMed Central
Year
2008
Tongue
English
Weight
165 KB
Volume
8
Category
Article
ISSN
1471-2210

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✦ Synopsis

Background

Norfloxacin like other fluoroquinolones, is known to be mutagenic for Salmonella typhimurium TA102 strain. This mutagenic effect is due to free oxygen radicals (ROS), because it is inhibited by antioxidants such as β-carotene and naturally occurring antioxidants of Roheo discolor and other plants. The aim of this work was to evaluate combination therapy with norfloxacin and vitamins C and E, to reduce the possible genotoxic risk associated with fluoroquinolones.

Method

The antimutagenicity of α-tocoferol (Vitamin E) and ascorbic acid (Vitamin C) against norfloxacin-induced mutation was evaluated on S. typhimurium TA102, using the aroclor-1254-induced S9 rat liver homogenate. The minimum inhibitory concentration (MIC) a measure of the bactericidal effect of norfloxacin, was obtained in vitro by the plate dilution method.

Results

Vitamin E (0.5 mg per Petri dish) induced a statistically significant reduction (P < 0.001) in the mutagenicity of norfloxacin, whereas Vitamin C (1 mg per Petri dish) had no such effect. Neither of these vitamins altered the MIC for norfloxacin against 25 uropathogenic strains of Escherichia coli.

Conclusion

These results suggest that Vitamin E is a potent antimutagen that would be worthwhile being used in conjunction with fluoroquinolone treatment. The minimal antimutagenic effect of Vitamin C observed under these experimental conditions may have been because Vitamin C in the Ames test induces a Fenton reaction, and if divalent cations are present, it can act as a pro-oxidant rather than an antioxidant. Ascorbic acid should be further evaluated in the presence of different divalent cations concentrations.

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