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Comparative study of freeze-substitution techniques for x-ray microanalysis of biological tissue

✍ Scribed by Eva Påsgård; Ulf Lindh; Godfried M. Roomans

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
511 KB
Volume
28
Category
Article
ISSN
1059-910X

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✦ Synopsis

A comparative study of some aspects of freeze-substitution as a preparative method for X-ray microanalysis of diffusible ions in biological specimens was carried out. Four substitution fluids were compared with each other and with cryosections. As criteria, elemental ratios as determined by X-ray microanalysis of thin sections of mouse pancreas were used. Freezesubstitution for 3 weeks in diethyl ether or for 2 days in tetrahydrofurane gave results comparable to those obtained in cryosections. Slow warming to room temperature after freeze-substitution in tetrahydrofurane gave slightly better results than fast warming. Freeze-substitution in acetone resulted in noticeable loss and redistribution of ions and freeze-substitution in methanol gave very poor results.

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