A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr
✦ LIBER ✦
Comparative study of conventional and novel strategies for the detection of hepatitis C virus RNA in serum: amplicor, branched-DNA, NASBA and in-house PCR
✍ Scribed by Françoise Lunel; Martine Mariotti; Pascale Cresta; Isabelle De La Croix; Jean-Marie Huraux; Jean-Jacques Lefrère
- Book ID
- 113256529
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 801 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0166-0934
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## Abstract The branched DNA (bDNA) assay was compared with a semi‐quantitative cDNA‐polymerase chain reaction (cDNA‐PCR) assay for monitoring HCV RNA levels in plasma in 17 haemophilia patients participating in a controlled a‐interferon trial. Good correlation between the HCV RNA levels as detecte