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Comparative study of conditions for demonstrating receptors of memory T cells and other T lymphocyte subpopulations immune to antigens of the H-2 complex

✍ Scribed by A. A. Pimenov; I. F. Abronina; B. D. Brondz


Publisher
Springer US
Year
1984
Tongue
English
Weight
267 KB
Volume
97
Category
Article
ISSN
0007-4888

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Expression of the T-cell receptor (TCR) zeta chain in normal individuals was studied by two-color flow cytometric analysis using digitonin-permeabilized human peripheral blood lymphocytes. Optimal detection of the TCR zeta chain involved fixation of cells in 0.25% paraformaldehyde for 2 min and perm