Comparative studies on the interaction of benzpyrene with cells derived from poikilothermic and homeothermic vertebrates. II. Effect of temperature on benzpyrene metabolism and cell multiplication
✍ Scribed by H. Fred Clark; Leila Diamond
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 583 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
The effect of incubation temperature on cell multiplication and on the efficiency of benzpyrene (BP) metabolism to water-soluble derivatives was compared in cell cultures derived from three poikilothermic and three homeothermic vertebrate species. The fish cells grew optimally at about 20°C and the amphibian and reptilian cells at about 30"C, and in general, these cells multiplied over broader ranges of temperature than the mouse, hamster or chick cells. In each cell system, the maximum temperature supporting efficient BP metabolism exceeded the maximum temperature supporting cell growth by 4 to 8 " , but the range of temperatures supporting near-maximal BP metabolism was also considerably broader in the poikilothermic than in the homeothermic vertebrate cultures.
We have previously characterized the capacity of phylogenetically diverse cell culture systems to metabolize the carcinogenic hydrocarbon, benzo( a)pyrene (BP), to water-soluble derivates (Diamond and Clark, '70). Cells from poikilothermic vertebrates, like cells from mammalian species (Andrianov et d., '67; Diamond, '70, '71), ranged from high to very low efficiency in their ability to degrade BP. The metabolism of the hydrocarbon in the different species was compared, with one exception, at a single standard temperature (33"C), although this was not necessarily the optimal temperature for cell growth. The poikilothermic cells were established in culture at incubation temperatures ranging from 23-36°C and vary in their capacity for growth at high temperatures (Wolf and Quimby, '62; Wolf et al., '66; Clark and Karzon, '67a,b; Zeigel and Clark, '69; Freed et al., '69; Clark, Cohen and Karzon, '70). Therefore, we selected three poikilothermic cell lines that do have the capacity to metabolize BP and studied the effect of temperature on the efficiency of BP metabolism and on the growth and sur-