Comparative studies by cell culture andin vivo implantation test on the toxicity of natural rubber latex materials
✍ Scribed by Ikarashi, Yoshiaki ;Toyoda, Kazuhiro ;Ohsawa, Naomi ;Uchima, Takao ;Tsuchiya, Toshie ;Kaniwa, Masa-Aki ;Sato, Michio ;Takahashi, Michihito ;Nakamura, Akitada
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 798 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Colony assay using V79 cells, the agar diffusion assay w i t h L929 cells, and the 7-day rabbit muscle implantation test were employed to evaluate the cytotoxicity and tissue toxicity of natural rubber latex (NRL) materials. The in vivo implant a t i o n t e s t s h o w e d t h a t , a m o n g 1 3 histological parameters, thickness of inflammatory layer was the most useful index to evaluate tissue responses quantitatively. A comparison of the in vivo and in nitro parameters revealed the following correlations between the thickness of the inflammatory layer and cytotoxicity indices: Colony assay of the extracts, ICs0: Y = 0.80; Agar diffusion assay, Zone index: I = 0.73; Lysis index: r = 0.61.
From these results, it appears that the colony assay provides a more reliable prediction of the tissue response than the agar diffusion assay.