𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Comparative screening for ciliary neurotrophic activity in organs of the rat and chicken

✍ Scribed by Dr. T. Ebendal

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
577 KB
Volume
17
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

A neuron-survival assay with dissociated ciliary ganglion neurons was used to examine the level of neurotrophic activity in organs of the rat and chicken. All tissue homogenates enhanced neuron survival at increasing protein concentrations, although 50% survival activity (1 trophic unit, TU, per ml) occurred at distinctly different levels in the different organs. Liver, spleen, T-cells, and submandibular gland from the rat were very low in survival-promoting activity (0.1-1.1 TU per mg of protein). Serum and lung were slightly richer (2.4-2.8 mg). Heart, brain, and skeletal muscle stimulated survival well (6.2-13 TU/mg). Unexpectedly, the highest activities were found in the rat kidney (44 TU/mg). The activity in adult chicken organs was less varied (1.6-9.1 TU/mg). Higher trophic activities were found in the chicken embryo, particularly at day 18 of development (20-30 TU/mg). The up to 100-fold difference in trophic level between organs should make it feasible to use subtractive hybridization, differential screening, and transient expression in eukaryotic cells for molecular cloning of the ciliary neurotrophic factor

πŸ“œ SIMILAR VOLUMES

Lesion-induced changes in the expression
Lesion-induced changes in the expression of ciliary neurotrophic factor and its receptor in rat optic nerve
✍ Matthias Kirsch; Thomas Schneider; Mun-Yong Lee; Hans-Dieter Hofmann πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 523 KB

There is evidence that ciliary neurotrophic factor (CNTF) is involved in reactive changes following lesions of the nervous system. To investigate, whether differences in the regulation of CNTF and CNTF receptor alpha (CNTFRalpha) contribute to the differences in PNS and CNS responses to injury, we h

Ciliary neurotrophic factor induces expr
Ciliary neurotrophic factor induces expression of the IGF type I receptor and FGF receptor 1 mRNAs in adult rat brain oligodendrocytes
✍ Fengjun Jiang; Steven W. Levison; Teresa L. Wood πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 744 KB

Ciliary neurotrophic factor (CNTF) is produced and released in response to injury in the central nervous system (CNS). While CNTF initially was characterized as a trophic factor for neurons, more recent evidence supports roles for this factor in survival, proliferation, and maturation of oligodendro

Long-term protection of axotomized neuro
Long-term protection of axotomized neurons in the dorsal lateral geniculate nucleus in the rat following a single administration of basic fibroblast growth factor or ciliary neurotrophic factor
✍ Agarwala, Seema; Kalil, Ronald E. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 459 KB πŸ‘ 2 views

We have studied the long-term effects of basic fibroblast growth factor (bFGF) and ciliary neurotrophic factor (CNTF) on axotomy-induced cell death in the dorsal lateral geniculate nucleus (LGN) of adult rats. LGN neurons were axotomized by a visual cortex lesion in 31 adult rats. A gelatin sponge s

Enzyme systems in Tetrahymena geleii S.
Enzyme systems in Tetrahymena geleii S. II. Acetylcholinesterase activity. Its relation to motility of the organism and to coordinated ciliary action in general
✍ Seaman, Gerald R. ;Houlihan, Robert K. πŸ“‚ Article πŸ“… 1951 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 650 KB

In the course of a general survey of enzyme systems in the ciliate Tcltrahyinena geleii S, it was found that the organism contains a n active acetylcholinesterase (ACliE) system. It was thought that this was the first demonstration of the system in a protozoan. A search of the literature, however, r