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Comparative porcine gene mapping relative to human chromosomes 9, 10, 20 and 22

✍ Scribed by J. H. Lee; W. Zhang; C. Moran

Book ID: 104457426
Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 63 KB
Volume: 32
Category: Article
ISSN: 0268-9146
DOI: 10.1046/j.1365-2052.2001.00787.x

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✦ Synopsis

Comparative anchor tagged sequence (CATS) consensus primers from loci mapped to human chromosomes 9, 10, 20, and 22 have been used to amplify homologous loci in pigs. Of 53, CATS primers tested in pigs, only 23 yielded products homologous to the human locus (42% success). Ten loci were physically mapped (43% success rate for verified products, but only 19% for primers tested). Due to lack of polymorphism, linkage mapping was possible only for AMBP. Map locations were consistent with human/pig ZOO‐FISH, except for ADRA1A, whose position is still equivocal in humans. These CATS primers have made very limited contributions to pig/human comparative gene mapping because of low efficiency of amplification of orthologous porcine product, frequent amplification from rodent template in a somatic hybrid panel and low level of polymorphism.

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