Comparative in vivo mutagenicity testing by SCE and micronucleus induction in mouse bone marrow

✍ Scribed by Th. Bauknecht; W. Vogel; U. Bayer; D. Wild

• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 580 KB
• Volume: 35
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00446622

✦ Synopsis

The treatment of mice with repeated injections of BUdR and FUdR allows for the demonstration of differentially stained metaphases from bone marrow after FPG (fluorescence plus Giemsa; Perry and Wolff, 1974) treatment. Thus, it is possible to determine the number of SCE's under in vivo conditions, which appears as a very promising system for mutagenieity testing. We studied the response of this system in comparison to the micronucleus test using six mutagenic agents: triaziquone, cyclophosphamide (CP), dimethylphenyltriazene (PDMT), methylnitro nitrosoguandine (MNNG), dimethylnitrosamine (DMNA), and diethylnitrosamine (DENA). With the exception of MNNG and DENA, all these agents induce both, SCE and micronuclei, MNNG and DENA being ineffective in both systems. The most potent SCE-inducing agent was triaziquone, followed by PDMT, CP, and DMNA. The quantitative comparison indicates that SCE are induced at 1/10--1/100 of the concentrations which are required for the detection of micronuclei.