Comparative in vivo antioxidant capacity of DL-2-hydroxy-4-methylthiobutanoic acid (HMTBA) and DL-methionine in male mice fed a high-fat diet
✍ Scribed by Xue Tang; Yonglan Yang; Yonghui Shi; Guowei Le
- Book ID
- 102441276
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 112 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Background:
In animal diets, methionine (met) is considered to be the first limiting amino acid, and the activity of synthetic met is typically added either as dl-methionine (dlm) or as dl-2-hydroxy-4-methylthiobutanoic acid (hmtba). it has been demonstrated that hmtba exhibits a higher antioxidant capability in vitro as compared to dlm. however, the difference in antioxidant capability between dlm and hmtba in vivo is unknown.
Methods:
In the present study, 60 male c57bl/6 mice were randomly divided into six groups and fed either a normal diet (nfd, 5.37% fat) or a high-fat diet (hfd, 19.7% fat) in conjunction with 0.2% dlm, 0.2% hmtba or 0.1% dlm and 0.1% hmtba for 4 weeks.
Results:
Hfd supplemented with 2% dlm and nfd with 2% hmtba both induced adverse affects in relation to serum lipid parameters and depressed antioxidant defense systems in the digestive system. however, these changes were restored in the 0.2% hmtba-treated hfd group. furthermore, no significant differences were found in the lipid parameters and antioxidant status in the nfd and hfd group supplemented with 0.1% dlm and 0.1% hmtba.
Conclusion:
Hmtba restored oxidative redox status under os conditions and its antioxidant properties were positively correlated with the dosage included in diet.