Comparative in vitro sensitivity of two methylcholanthrene-induced murine sarcoma lines to humoral and cellular immune cytotoxicity
✍ Scribed by Georges Bataillon; Hugh Pross; George Klein
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- French
- Weight
- 804 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
MC57M and G are the tissue‐culture derivatives of two methylcholanthreneinduced murine C57Bl sarcomas. Their sensitivity to immune cytotoxicIn this report, the term “cytotoxicity”, unless otherwise qualified, will be used in the generic sense, covering such various in vitro effects of immune effectors as true cytotoxicity (actual reduction in the number of target cells), cytostasis (inhibition of target‐cell growth) and cytolysis. Clearly, other phenomena yet to be explored, such as cell adhesiveness or cell membrane permeability to isotopically‐labelled products, may well be involved in and interfere with the final outcome of some of the assays used in this work.
, cytostatic or cytolytic spleen cells and sera was compared in parallel in vitro assays. The level of cross‐reactivity displayed by the two lines was found to depend on the nature of the immune effector rather than on the assay which was used. It was high with immune spleen lymphocytes, alone or in the presence of decomplemented antisera, and low with antisera in the presence of rabbit complement. MC57G cells were more sensitive than MC57M cells to both effectors. Both cell lines were insensitive to antibody‐dependent cell‐mediated cytotoxicity. Preliminary evidence is presented, suggesting a probable involvement of embryonic and Moloney leukemia virus‐induced cell surface antigens in the in vitro sensitization of the two tumor lines to immune sera.