Comparative immunochemical study of ribosomal proteins from various mammalian cells by two-dimensional immunoelectrophoresis

✍ Scribed by Auger-Buendia, Marie-Annick ;Ollier-Hartmann, Marie-Paule ;Tavitian, Armand ;Hartmann, Lucien

• Book ID: 104769270
• Publisher: Springer
• Year: 1981
• Tongue: English
• Weight: 811 KB
• Volume: 182
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00422785

✦ Synopsis

Proteins isolated from ribosomal subunits of various mammalian cells were analysed comparatively by two different methods: a two-dimensional polyacrylamide gel electrophoresis system and a recently described two-dimensional immunoelectrophoresis technique. For this purpose, antisera were raised in rabbits against the total mixture of ribosomal proteins from murine cells. These sera were characterized by ring-test, double immunodiffusion and two-dimensional immunoelectrophoresis. They were shown to contain antibodies to a large number of ribosomal proteins. Immunoelectrophoretic analysis of 60S and 40S subunit proteins from rabbit, lamb, canine and human cells using anti-murine sera revealed a striking conservation of their antigenic properties. These results corroborated those obtained by two-dimensional polyacrylamide gel electrophoresis.