Comparative expression study to increase
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Laila Niiranen; Sigrun Espelid; Christian R. Karlsen; Milla Mustonen; Steinar M.
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Article
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2007
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Elsevier Science
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English
β 277 KB
Functional and structural studies require gene overexpression and purification of soluble proteins. We wanted to express proteins from the psychrophilic bacterium Vibrio salmonicida in Escherichia coli, but encountered solubility problems. To improve the solubility of the proteins, we compared the e