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Comparative expression study to increase the solubility of cold adapted Vibrio proteins in Escherichia coli

✍ Scribed by Laila Niiranen; Sigrun Espelid; Christian R. Karlsen; Milla Mustonen; Steinar M. Paulsen; Pirkko Heikinheimo; Nils P. Willassen


Book ID
116828486
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
277 KB
Volume
52
Category
Article
ISSN
1046-5928

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Comparative expression study to increase
✍ Laila Niiranen; Sigrun Espelid; Christian R. Karlsen; Milla Mustonen; Steinar M. πŸ“‚ Article πŸ“… 2007 πŸ› Elsevier Science 🌐 English βš– 277 KB

Functional and structural studies require gene overexpression and purification of soluble proteins. We wanted to express proteins from the psychrophilic bacterium Vibrio salmonicida in Escherichia coli, but encountered solubility problems. To improve the solubility of the proteins, we compared the e