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Comparative evaluation of Abbott and Murex antibody assays and Roche Amplicor PCR for diagnosis of human hepatitis C

✍ Scribed by D. Liu; J. Steele; S. Lloyd Jones; D. Leslie; J. Pedersen; R. Baird


Book ID
114268922
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
357 KB
Volume
8
Category
Article
ISSN
0888-0786

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A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr