Comparative evaluation of [99mTc(H2O)3(CO)3]+ precursor synthesized by conventional method and by using carbonyl kit

✍ Scribed by Drishty Satpati; Madhav Mallia; Kanchan Kothari; M.R.A. Pillai

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: French
• Weight: 261 KB
• Volume: 47
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.852

✦ Synopsis

Abstract

A comparison of the preparation of Tc carbonyl complexes of a few ligands of interest through the conventional method as well as by using the recently introduced carbonyl kit method is described. Synthesis of [^99m^Tc(OH~2~)~3~(CO)~3~]^+^ precursor was carried out by the conventional method in which ^99m^TcO was reduced with sodium borohydride and carbonylated by purging CO gas. Tricarbonyl complexes of MIBI, TBI, isoniazid and mebrofenin were prepared using the above precursor under optimized conditions. [^99m^Tc(OH~2~)~3~(CO)~3~]^+^ precursor was also synthesized by using the carbonyl kit and its utility for the synthesis of tricarbonyl complexes was studied by carrying out synthesis of complexes with the above ligands. For comparison, these complexes were first prepared under similar conditions optimized with conventional precursor in order to see the variation in the yields in the two different procedures. Characterization of the precursors as well as the respective tricarbonyl complexes was carried out by reverse phase HPLC using C18 column. It was observed that [^99m^Tc(OH~2~)~3~(CO)~3~]^+^ precursor could be synthesized in high yields using the carbonyl kit, however, the reaction kinetics was found to be slow while using the kit precursor. In the case of TBI and MIBI, it was observed that increase in reaction time was essential to achieve good yields. A five‐fold increase in the ligand concentration was needed while using the carbonyl kit in order to get good complexation yields with isoniazid and mebrofenin. Copyright © 2004 John Wiley & Sons, Ltd.