Comparative differentiation and numeration of CFUs from mice infected either by the anemia- or polycythemia-inducing strains of friend viruses
✍ Scribed by F. Wendling; P. Tambourin; O. Gallien-Lartigue; M. Charon
- Book ID
- 102867000
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- French
- Weight
- 657 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
A comparative study of alterations in the CFU (colony‐forming unit) compartment of susceptible mice infected either with the anemia‐ (FVA) or the polycythemia‐inducing Friend virus (FVP) indicated that: (1) Whatever the type of virus used, the absolute number of CFU is increased in the spleen and in the blood; (2) The splenic CFU augmentation parallels the increase in cellularity so that the CFU concentration remains equal to that of control spleens; (3) Circulation of the CFU in the peripheral blood is multiplied by a factor of about 100 in FVA‐infected mice and 500 in FVP‐infected mice 25 days after infection; (4) In the femoral bone marrow, the CFU number is either unaltered (FVP infection) or decreased (FVA infection). Stem cells from leukemic mice are able to give rise to colonies of differentiated hematopoietic cells as do control CFU. The distribution of the various types of colonies is similar between the different tissues and the different groups of donors. Despite considerable differences between FVA and FVP pathological erythropoiesis, no qualitative discrepancies are observed between the population sizes and the differentiating abilities of the pluripotential stem‐cell compartment. The increase in number of CFU appears to be incidental to the oncogenic action of the virus at the level of the erythroid precursor cells.