Comparative cytogenetic analysis between cyclophosphamide-sensitive and -resistant lines of acute myeloid leukemia in the lewis brown norway hybrid rat
✍ Scribed by Dr. William Kearns; Todd Koelling; Andrew Yeager
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 536 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
An animal model of acute myeloid leukemia (AML) has been developed in the Brown Norway (BN) rat and has successfully been introduced into the Lewis X BN F, hybrid (LBN) and designated LBN AML. The original LBN AML is sensitive t o the chemotherapeutic agent cyclophosphamide (CY). Recently, a CY-resistant cell line of LBN AML has been established. To characterize this animal model of human leukemia better, we analyzed and compared the chromosomal makeup of both the CY-sensitive and CY-resistant LBN AML lines. The CY-sensitive LBN AML cultures contained two cell lines-line I (88%): 41 ,XX,-I ,-2,-9,del( I2)(q 16),+der( I ) t ( 1;?8)(pI3;q3l),+der(2)t(2;9)(pl I;ql I); and line I 1 (I 2%): 41,XX,-I , -2,-9,del( l2),del(20)(q I3)+der( I)t( 1;?8)(pI3;q3I),+der(2)t(2;9)(pI l;ql I). The recently developed CY-resistant AML cells contained t w o cell lines-line I (88%): 4 I ,XX,-I ,-2,-9,de1(3)(q36q42. I),del(4)(q42.2),?t(5;?)(q35;?), ?t(8?)(q24;?),del(I2)(qlb),+der (l)t(l;?8)(pl J;q3I),+der(Z)t(2;9)(pl I;ql I); and line I\ (12%): 42,XX (probably represents host contamination). The new chromosomal aberrations in the CY-resistant line I [de1(3)(q36q42. I),del(4)(q42.2),?t(5;?)(q35;!), and ?t(8?)(q24;?)] suggest a possible interrelationship between these secondary karyotypic abnormalities and acquisition of resistance t o the chemotherapeutic agent.