✦ LIBER ✦

Comparative cell targeting in vitro using the CO2 laser

✍ Scribed by Nojan Talebzadeh; Paul R. Morrison; Dr. Marvin P. Fried

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 450 KB
Volume: 14
Category: Article
ISSN: 0196-8092
DOI: 10.1002/1096-9101(1994)14:2<164::aid-lsm1900140209>3.0.co;2-g

No coin nor oath required. For personal study only.

✦ Synopsis

In keeping with current interest in bactericidal effects of lasers to treat localized infection, we examined the effect of C 0 2 laser light on three bacterial species. Cell monolayers were placed atop filters minimizing the presence of extracellular water. Sam les were exposed to irradiance values between 0 and 520 W/cm for 0.1 s. Cell survival was determined by colony counts. There was logarithmic killing of the bacteria with increasing irradiancei 90% kill was effected at 180 W/cm2 for Escherichia coli, 350 W/cm for Streptococcus mutans, and 250 W/cm2 for Bacillus stearothermophilus. However, standard deviations suggest no significant difference between cell types. Our results show the bactericidal effect of the COP laser with no remarkable difference between Gram types. However, substantial cell kill was achieved at relatively low irradiance. Whereas selectivity of damage may not be afforded by the COP laser, it may minimize collateral damage.

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