Comparative biochemistry of human skin: glycosaminoglycans from different body sites in normal subjects and in patients with localized scleroderma
✍ Scribed by CO Passos; CC Werneck; GR Onofre; EA Pagani; AL Filgueira; L-CF Silva
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 369 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0926-9959
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✦ Synopsis
ABSTRACT
Objectives The aim of this investigation is to compare the relative proportions of disaccharides of chondroitinase‐digestible glycosaminoglycans (GAGs) among the different body sites in control human skin and in the skin lesions of patients with localized scleroderma.
Methods The disaccharide relative proportions were determined using high‐performance liquid chromatography (HPLC).
Results ΔDi‐4S, the main disaccharide unit of dermatan sulphate (DS), was the major skin GAG disaccharide (∼70% of the total) in control skin among all different body sites studied here. In scleroderma there was an increase in the relative proportion of both ΔDi‐HA, the main disaccharide unit of hyaluronic acid (HA), and ΔDi‐diS~B~ (α‐ΔUA(2SO~4~)‐1→3‐GalNAc(4SO~4~)), derived from DS, and a decrease in ΔDi‐4S, as compared with the uninvolved skin or the site‐matched control skin.
Conclusion DS is the major GAG species in normal skin from different body sites. In addition, our results suggest a decrease and also a structural change in DS and an increase in the proportion of HA in scleroderma skin.