✍ Scribed by Carlo Giannini; Valérie Thiers; Jean-Baptiste Nousbaum; Lieven Stuyver; Geert Maertens; Christian Bréchotl
No coin nor oath required. For personal study only.
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Group I and II hepatitis C virus genotypes were determined by a newly developed serological genotyping assay. This assay detected antibodies against group-specific recombinant proteins in the putative NS4 pro\*in region (amino acid no. 1676-1760) by an enzyme-linked immunosorbent assay. This region
Methods for identifying the genotype of hepatitis C virus (HCV) in clinical specimens are frequently based upon the direct characterisation of viral RNA sequences by polymerase chain reaction (PCR) amplification, or by serologically based methods, in which the infecting genotype is inferred from the
## Abstract A polymerase chain reaction (PCR)‐based assay using primers against the hepatitis C core gene has been described [Okamoto et al. (1992a): __Journal of General Virology__ 73:673–679]. Within the two major HCV genotypes 1 and 2, the Okamoto system identifies two subtypes each (1a,1 b and