Comparative analysis of a large panel of non-starch polysaccharides reveals structures with selective regulatory properties in dendritic cells
✍ Scribed by René Wismar; Susanne Brix; Helle Nygaard Lærke; Hanne Frøkiær
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 416 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1613-4125
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✦ Synopsis
Structural-based recognition of foreign molecules is essential for activation of dendritic cells (DCs) that play a key role in regulation of gut mucosal immunity. Orally ingested non-starch polysaccharides (NSP) are ascribed many health-promoting properties, but currently we lack insight into the impact of structure and size for their capacity to affect immune responses. Methods and results: This study addresses the importance of chemical structure, size, origin and presence of contaminants for the capacity of both dietary and non-food NSP to modulate DC. Of 28 NSP products, b-glucans of microbial and plant origin and the galactomannan guar gum were found to modulate the DC cytokine pattern induced by the Toll-like receptor 4-ligand LPS giving rise to reduced IL-12p70 and increased IL-10 levels, whereas IL-6 production was unaffected. A large proportion of the tested NSP were able to down-regulate LPS-induced IL-12p70 production. The most potent NSP induced up-regulation of CD86 on DC independently of LPS stimulation. Cereal-based b-glucans showed less potency than bglucans of microbial origin, but proper molecular weight composition and preparation may improve effectiveness. Conclusions: Collectively, this comparative study revealed that some plant-derived NSP besides those of microbial origin exert modulation of the DC phenotype, with the exact structure being important for the activity.