Common conditions for high-performance liquid chromatographic microdetermination of aldoses, hexosamines, and sialic acids in glycoproteins

✍ Scribed by Susumu Honda; Shigeo Suzuki

• Book ID: 102987165
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 581 KB
• Volume: 142
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90533-5

✦ Synopsis

All aldoses known to be present in annnal and plant glycoproteins were separated in partition mode on a column of a highly crosslinked cation-exchange resin (Shodex DC-613, H+ form) and sensitively monitored by photometric detection after postcolumn labeling with 2cyano-a&amide. Linearity for the sample amount ranging from 0.1 to 80 nmol was observed with high reproducibility. Hexosamines could be determined as their N-acetyl derivatives under the identical conditions with approximately one order of magnitude higher range of linearity. Sialic acid could be estimated as N-acylhexosamines produced by the action of N-acetylneuraminate pyruvate-lyase. The usefulness of these common conditions was demonstrated by analyzing component monosaccharides of some glycoprotein preparations. Q 1984 kadcmic m inc.

KEY WORDS: monosaccharide composition analysis of glycoproteins; high-performance liquid chromatography of aldoscs, hexosamines, and sialic acids; analytical application of Nacetylneuraminate pyruvate-lyase.